Exploring the Meaning of Ou in Greek
οὐ means “no” and appears 1,693 times in Scripture, including Matthew 1:25; 2:18; 3:11; 4:4, 7; 5:14, 17–18.
Matthew Examples
In Matthew it appears in statements like “didn’t know” (1:25) and “shall not” (4:4; 4:7).
Learn More →οὐ expresses denial—“no”—and in these passages it regularly frames what is refused, prohibited, or ruled out. Across the sampled scenes in Matthew, it shapes narrative statements, prophetic lament, and Jesus’ authoritative teaching by marking a clear negation.

Occurrences
Matthew 1:25: “and didn’t know her sexually until she had given birth to her firstborn son. He named him Jesus.”
Here οὐ negates an action in a narrative summary: Joseph “didn’t know her sexually” during a defined span (“until she had given birth”). The negation sets a boundary around the relationship prior to the birth and gives the reader a plain denial of that act in the time specified.

Matthew 2:18: ““A voice was heard in Ramah, lamentation, weeping and great mourning, Rachel weeping for her children; she wouldn’t be comforted, because they are no more.””
In this lament, οὐ supports two denials embedded in grief. First, “she wouldn’t be comforted” presents comfort as something refused or impossible to receive in the moment of mourning. Second, “they are no more” uses negation to state the children’s absence with finality, intensifying the reason given for inconsolable weeping.
Matthew 3:11: “I indeed baptize you in water for repentance, but he who comes after me is mightier than I, whose sandals I am not worthy to carry. He will baptize you in the Holy Spirit.”
John’s comparison includes a self-assessment framed by negation: “I am not worthy to carry” the sandals of the mightier one. οὐ marks the denial of worthiness, sharpening the contrast between John’s role (“baptize you in water for repentance”) and the greater figure’s might and coming work.
Matthew 4:4: “But he answered, “It is written, ‘Man shall not live by bread alone, but by every word that proceeds out of God’s mouth.’ ””
οὐ appears in a scriptural citation that denies a sufficiency claim: a person “shall not live by bread alone.” The negation does not deny bread’s existence in human life; rather, it denies bread as the sole basis of life in the statement’s logic, turning attention to “every word that proceeds out of God’s mouth.”
Matthew 4:7: “Jesus said to him, “Again, it is written, ‘You shall not test the Lord, your God.’ ””
In another citation, οὐ carries a direct prohibition: “You shall not test the Lord, your God.” The negation functions as a boundary for conduct toward God, giving the command its firm, excluding force and making the prohibition explicit rather than implied.
Matthew 5:14: “You are the light of the world. A city located on a hill can’t be hidden.”
Here οὐ negates the possibility of concealment: a hilltop city “can’t be hidden.” The denial is presented as a matter of reality, not merely instruction; the negation supports the image by stating that such a city’s visibility resists being covered or kept out of sight.
Matthew 5:17: ““Don’t think that I came to destroy the law or the prophets. I didn’t come to destroy, but to fulfill.”
οὐ is central to Jesus’ clarification of purpose. It first blocks a mistaken conclusion (“Don’t think that I came to destroy”), then repeats the negation in an explicit denial (“I didn’t come to destroy”) before placing alongside it the contrasted aim (“but to fulfill”). The negation works to exclude one interpretation of his mission in order to secure another.
Matthew 5:18: “For most certainly, I tell you, until heaven and earth pass away, not even one smallest letter or one tiny pen stroke shall in any way pass away from the law, until all things are accomplished.”
This statement uses negation to assert permanence with precision: “not even one smallest letter or one tiny pen stroke shall in any way pass away.” οὐ strengthens the claim by denying even the smallest conceivable loss. The negation is paired with time markers (“until heaven and earth pass away… until all things are accomplished”), reinforcing the durability being affirmed by denying any diminishment.
Matthew 5:20: “For I tell you that unless your righteousness exceeds that of the scribes and Pharisees, there is no way you will enter into the Kingdom of Heaven.”
Here οὐ marks exclusion: “there is no way you will enter.” The negation does not merely warn of difficulty; it closes off entry under the stated condition (“unless your righteousness exceeds…”). The denial defines a threshold and makes the consequence categorical.
Matthew 5:21: ““You have heard that it was said to the ancient ones, ‘You shall not murder;’ and ‘Whoever murders will be in danger of the judgment.’”
In this recalled command, οὐ gives the prohibition its direct form: “You shall not murder.” The negation is imperative in effect, setting an absolute constraint on an action and presenting the command as a settled moral boundary within what has been “said to the ancient ones.”
Matthew 5:26: “Most certainly I tell you, you shall by no means get out of there, until you have paid the last penny.”
οὐ intensifies the certainty of non-release: “you shall by no means get out of there” before the condition is met (“until you have paid the last penny”). The negation establishes confinement as the default outcome and denies escape prior to full payment, giving the warning its strict, unavoidable shape.
Matthew 5:27: ““You have heard that it was said, ‘You shall not commit adultery;’”
Again, οὐ carries a prohibition, here directed at a specific act: “You shall not commit adultery.” The negation expresses the command as a definitive exclusion of the act from permitted behavior, presented as part of what the audience has “heard that it was said.”

Sense and Usage
Across these occurrences, οὐ consistently functions as a marker of denial that can operate in several recognizable ways without shifting away from its basic force. In narrative discourse it negates an action as a factual statement, as in “didn’t know her sexually” (Matthew 1:25), where the denial is bounded by “until,” and in “I am not worthy” (Matthew 3:11), where the denial is an evaluation about the speaker.
In poetic or prophetic lament it denies comfort and asserts absence: “she wouldn’t be comforted” and “they are no more” (Matthew 2:18). Here the negation is emotionally loaded, because what is denied is not merely an action but the possibility of consolation and the continued presence of children. The force of “no” becomes a way of stating loss in the starkest terms.
In Jesus’ teaching, οὐ often appears in formal prohibitions—“You shall not…” (Matthew 4:7; 5:21; 5:27)—where the negation serves as a boundary line. It also appears in declarative denials that reject an interpretation (“Don’t think… I didn’t come to destroy,” Matthew 5:17), and in statements denying possibility (“can’t be hidden,” Matthew 5:14). When paired with emphatic framing, it can express comprehensive exclusion: “not even one smallest letter or one tiny pen stroke shall in any way pass away” (Matthew 5:18) and “there is no way you will enter” (Matthew 5:20). In such contexts the negation is used not merely to say that something is untrue, but to close the door on alternatives and to define what cannot happen under the conditions stated.
The selection also shows οὐ functioning within contrastive structures: “shall not live by bread alone, but by every word…” (Matthew 4:4) denies a limiting claim in order to open a fuller one, and “not… to destroy, but to fulfill” (Matthew 5:17) uses negation to clear away one purpose so that another stands out. In these constructions, the negative particle plays a decisive role in shaping meaning by restricting one side of a contrast and thereby sharpening the other.
Imagery in Context
Several of these passages attach vivid images to negation. A hilltop city “can’t be hidden” (Matthew 5:14), where denial creates a picture of unavoidable visibility. The statement about the law—“not even one smallest letter or one tiny pen stroke shall in any way pass away” (Matthew 5:18)—uses negation to evoke something intact down to the smallest marks. Even the lament’s “she wouldn’t be comforted” and “they are no more” (Matthew 2:18) frames absence and refusal as images of grief that cannot be undone by ordinary consolations.
Sources: Lexical data from Strong’s Exhaustive Concordance and the Translators Brief Lexicon of Extended Strongs for Greek (STEPBible, CC BY). Occurrence data from the Translators Amalgamated Greek New Testament (STEPBible, CC BY). Scripture quotations from the World English Bible (public domain).





